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Royal College of Surgeons Ireland
Cellix, in collaboration with Dr. Gerardene Meade and Prof. Dermot Kenny, Molecular and Cellular Therapeutics from the Royal College of Surgeons Ireland (RCSI), put together a project which would use the VenaFlux Platform to mimic blood flow and monitor
thrombus formation.
Objectives
Investigate platelet adhesion and thrombus formation under physicological flow conditions using reduced volumes with Cellix's platform.
Equipment Used
- VenaFluxTM Platform
- Vena8 BiochipsTM
- Mirus NanopumpTM
- MetaMorph Imaging Software
Results
The rolling/adhesion of platelets on Vena8 biochips,
coated with adhesion molecule vwf, occurs at lower shear stress (4 and
40 dyne/cm), whereas at higher shear stress (60 and 120 dyne/cm2) platelet thrombus formation appears. A threshold limit of approximately 60 dyne/cm is required for thrombus formation, conditions that occur in microcirculation.
For more information please click here to view the Cardiovascular Application Note: Platelet - Thrombosis - Adhesion Assay -C100
For more information please click here to view the Cardiovascular Application Note: Platelet - Thrombosis - Adhesion Assay -C100
Acknowledgements
Cellix thanks Dr. Gerardene Meade and Prof. Dermot Kenny, Molecular and Cellular Therapeutics, Royal College of Surgeons in Ireland for carrying out the platelet adhesion studies.