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University College Dublin
In
collaboration with Prof. William Gallagher of the Conway Institute, UCD, Cellix
focused on a Melanoma research project mimicking a physiological relevant environment enabling
researchers to model survival in circulation and adhesion to endothelial
cell-derived proteins.
In this study, we determined if the steps in
extravasation differed in an isogenic melanoma cell line model of
progression.
Objectives
Melanoma is known as the most agressive form of skin cancer, which invades deeper layers of the skin and has the propensity to metastasise early. The aim of this study was to investigate the mechansim by which melanoma cells metastasise. Differential cell adhesions were examined within an isogenic model of melanoma progression under physiological shear flow conditions using Cellix Products.
Equiptment Used
Results
Results showed that shear stress plays an important role in the extravasation
process. While all cell lines investigated were highly adhesive under static
conditions, there was a surprising inability of some to adhere under flow with
the exception fo 1205Lus cells. These results may aid researchers to delineate
novel markers associated with extravasation which may serve as specific targets
for the treatment of cancer.
Acknowledgements
Cellix thanks Prof. William Gallagher and members of his research team; Dr Marin Rafferty and Ms. Gebriela Gremel for their expertise, generosity and time throughout this fruitful collaboration.