Back to Navigation
University of Washington
Cellix in collaboration with the University of Washington undertook a study in the
laboratory of Professor Evgeni Sokurenko at the University of Washington to
investigate bacterial pathogenesis in the Vena8 biochips, which represent an
advance in the approach to investigating and comprehending the mechanisms of
adhesion of bacteria.
Investigating invasiveness, colonization, and toxigenesis potential in real time in a dynamic environment will provide scientists with the know-how to identify novel therapeutics. This area of bacterial adhesion and biofilm culture also has applications in the field of cardiovascular stent infections.
Investigating invasiveness, colonization, and toxigenesis potential in real time in a dynamic environment will provide scientists with the know-how to identify novel therapeutics. This area of bacterial adhesion and biofilm culture also has applications in the field of cardiovascular stent infections.
Objectives
The main objective was to elucidate the importance of physiological shear stress environment required for E.coli adhesion, colonisation and biofilm formation using the Cellix VenaFluxTM Platform.
Equiptment Used
Results
- Shear Stress enhances accumulation of E.coli on surfaces coated with a 1M and 3M ligant. The bacteria adhered readily on the 1M and 3M surfaces at the lowest shear stress tested and accumulation increased with increased shear stress.
- Incrased shear stress results in a transitional from rolling to stationary E.coli ahdesion following the FimH-expressing E.coli accumulation to 1M and 3M ligands, the bacteria exhibit two adhesion modes:weak rolling adhesion and firm stationary binging. The rolling mode dominates at lower shears.
Achknowledgements
Cellix thanks Evgeni V.Sokurenko, Veronike Tchesnokova and Olga Yakovenko, Department of Microbiology University of Washington School of Medicine, Seattle, USA for experimental execution and collaboration.