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Webinar
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Cellix's free webinars series is an ongoing, informative program covering a wide range of topics. It allows you to gain an insight into current issues in the in-vivo market and how Cellix's products are used in different therapeutic research areas. In order to download previous webinars or gain access to our next upcoming webinar, you must REGISTER. |
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Our next upcoming webinar: Title: Inflammation: Leukocyte Adhesion Assays Date: 7th July, 2010 Time: 9:45 AM EST (New York) or 2:45 PM GMT (London) |
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Data are presented on adhesion profiles (cell recruitment) of monocytes and PBMCs on a range of adhesion molecules and endothelial cells including analysis of the role of these proteins and other chemokines involved in various stages of inflammation and leukocyte trafficking under physiological flow conditions. Assays were performed using Cellix’s VenaFlux Microfluidic Platform What you will learn: Results show that individual contributions of mAbs (anti-VCAM-1 mAb, anti-E-selectin mAb, anti-ICAM-1 mAb) does not equal the effect of all antibodes together, suggesting a compensatory effect when only one ligand on THP-1 or PBMCs is blocked; or the presence of a critical ligand cluster size for cell adhesion. MCP-1 stimulation was also investigated which showed to havea much stronger effect in the case of PBMCs isolated from blod compared to the THP-1 cell lines. These data support hypothesis of MCP-1 priming PBMCs for increased adhesion to inflamed endothelium under physiological flow conditions. |