3. VenaEC Biochip
The VenaEC Biochip contains 2 parallel microcapillaries that is mounted onto a tissue culture treated substrate which enables the seeding and culturing of endothelial cells. Cells derived from different endothelial tissues can be grown easily on the VenaEC Biochips. Validated examples to date include a Human Coronary Artery Endothelial Cell (HCAEC) monolayer for the investigation of monocyte adhesion for cardiovascular diseases and a Human Microvascular Endothelial Cell (HMVEC) monolayer for the investigation of eosinophils adhesion for respiratory diseases.
Features
- Suitable for a wide range of cell suspensions including T-cells, monocytes, neutrophils, eosinophils, platelets and whole blood (heparanized).
- Easy to seed and culture a variety of endothelial cells including HUVEC, HMVEC, HCAECs.
- Biochip plastic is optically clear permitting detailed microscopy studies.
- 0.05 to 100 dyne/cm2 shear stresses easily obtained and controlled by the Mirus 1.0 Nanopump.
- Shear stress may be preset to incrementally increase during an assay.
- Real-time imaging under flow conditions.
VenaEC Biochip
GFP-labelled MDA (breast cancer) cells on HUVEC cell line in VenaEC Biochip
Monocytes on HCAEC monolayer in VenaEC Biochip
Performance Specifications
| Cell Suspension Assay: | |
| Minimum Sample Volume | 10µl |
|---|---|
| Shear Stress Range* | 0.05 – 20 dyne/cm2, steps of 0.05dyne/cm2 |
| Volumetric Flow Rates | 100 nL/minute – 20 µL / minute |
| Sample Volume Aspiration Accuracy | ± 1% |
| Shear Stress Accuracy | ± 0.5% |
| Sample Volume Aspiration Precision < 1% CV | |
| Shear Stress Precision | < 0.5% CV |
*Utilising Cellix’s Mirus Nanopump.
Technical Specifications
| Number of channels per biochip | 2 |
|---|---|
| Number of substrates per biochip | 4 |
| Volume of each channel | 0.8 µL |
| Height of channel | 120 µm / 0.12mm |
| Width of channel | 600 µm / 0.6mm |
| Length of channel | 20mm |
| Dead volume at input port | 0.1 µL |
| VenaEC biochip dimensions | 600 µm (W) x 120 µm (D) x 20 mm (L) |
Downloads
- VenaEC Biochip Brochure (.pdf 1658 KB)